Human Single-Cell Suspensions

ProteoGenex has extensive experience in providing isolated human primary cells derived from fresh tissues (solid tumors and normal tissues).

  • After resection, tissues are kept fresh on ice until dissociation.
  • Tissues are then macro-dissected with sterile razors and dissociated in collagenase solution for 2-8 hours at 37°C.
  • Digested cells are pelleted by centrifugation (1200 rpm for 8 minutes).
  • The pellet is washed, spun, and resuspended 3 times in 10mL RPMI1640 and 15% FBS.
  • Isolated primary cells are resuspended in freezing media and slowly cooled to -80°C.
  • Approximately 1 x 107 cells can be isolated from each tissue specimen.
  • Single cell suspension vials are stored in vapor phase liquid nitrogen (-190°C).

Call or email our team to discuss your custom project or ask about specimen availability in our extensive biorepository.