Human Single-Cell Suspensions

ProteoGenex has extensive experience in providing isolated human primary cells derived from fresh tissues (solid tumors and normal tissues).

• After resection, tissues are kept fresh on ice until dissociation.
• Tissues are then macro-dissected with sterile razors and dissociated in collagenase solution for 2-8 hours at 37°C.
• Digested cells are pelleted by centrifugation (1200 rpm for 8 minutes).
• The pellet is washed, spun, and resuspended 3 times in 10mL RPMI1640 and 15% FBS.
• Isolated primary cells are resuspended in freezing media and slowly cooled to -80°C.
• Approximately 1 x 10⁷ cells can be isolated from each tissue specimen.
• Single cell suspension vials are stored in vapor phase liquid nitrogen (-190°C).