Do you collect biospecimens from consented donors only?
How do you protect donor’s confidentiality?
Do you have (IRB/IEC) approval for samples collection?
Fresh Frozen Tissue Samples
What is fresh frozen tissue sample size/weight?
What is the time between surgery/biopsy and tissue samples freezing?
What QC do you perform for fresh frozen tissue samples?
H&E slides preparation from each fresh frozen tissue sample by independent pathologists for tissue evaluation and diagnosis confirmation.
Extraction of total RNA is performed on all fresh frozen samples collected for ProteoGenex. RNA is analyzed using the Agilent 2100 Bioanalyzer.
Clinical data QC:
All data are carefully reviewed for accuracy and completion.
Do you prepare FFPE blocks with matching fresh frozen tissue samples?
What samples can you collect from a single patient?
What human biofluid samples can you collect?
Plasma, serum, whole blood collected in PAXgene RNA and DNA tubes, BM, urine, saliva, sputum, amniotic fluid, CSF, etc.
Can you perform samples collection in client's tubes?
ProteoGenex is able to perform collection using client’s collection tubes/kits.
Do you collect viable cells from blood or BM?
Separation and freezing down of viable WBC are performed using ProteoGenex SOP or SOP provided by clients.
Do you collect samples from patients with hematological malignancies?
- Leukemia: ALL, CLL, AML, CML
- Lymphoma: NHL, Hodgkin’s disease
- Multiple myeloma
- Myelodysplastic syndrome
Blood/BM samples can be collected at different stages of a disease.
What method of fixation do you use for FFPE blocks preparation?
All FFPE tissue samples are fixed in 10% neutral-buffered formalin within 30 minutes of surgery excision and embedded in IHC-grade paraffin.
What is a standard FFPE block sample size?
Standard FFPE block size is 1x1x0.5 cm, but can vary depending on the nature of a disease and tissue type.
What information do you provide for FFPE blocks?
Each FFPE block is accompanied with the following information:
- ID #
- Histological diagnosis
- Differentiation grade
- Location of distant metastasis
- Number of analyzed lymph nodes
- Number of positive lymph nodes
- Tumor size
- Date of surgery
- Treatment history (if applicable)
Do you perform IHC staining?
We provide IHC services on human tissues to identify specific cell types in which your target proteins are expressed.
Do you prepare TMA blocks?
ProteoGenex provides custom-built Tissue Microarrays.
What are core sizes for TMA blocks you provide?
Core sizes are 0.6 mm, 1 mm, and 2 mm.
How many cores per one block can you prepare?
Up to 400 cores per TMA block.
Do you provide blocks with follow-up information?
We can prepare blocks or cut sections from blocks with up to 5 years follow-up information.